| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | The cells were seeded in 60-mm dishes and cultured with or without 1 μg/ml APN (Z03072; Genscript, Piscataway, NJ, U.S.A.) for 7 days to examine ossification-related gene expression or for 21 days to assess nodule formation by Alizarin Red S staining...The cells (5000 cell/cm2) were seeded in 60-mm dishes for 24 h and cultured with or without 1 μg/ml APN (Z03072; Genscript, Piscataway, NJ, U.S.A.) for 7 days to examine osteogenic-related gene expression or for 21 days to assess nodule formation by Alizarin Red S staining. | Get A Quote |
Periodontal diseases are infections of the structures that surround and support the teeth; they are characterized by local inflammation and alveolar bone loss. Most treatments focus on only one aspect, inhibiting inflammation, or promoting osteoblasts. We set out to develop a new method that would intervene in the two aspects simultaneously. Adiponectin (APN), secreted by adipocytes, inhibits the inflammatory response and promotes osteogenesis. However, its role in human periodontal ligament cells (hPDLCs) is unclear. Therefore, we aim to investigate whether APN could suppress lipopolysaccharide (LPS)-induced inflammation and promote osteogenesis in hPDLCs. In the present study, we stimulated hPDLCs with LPS in... More
